Hhmi-gcat

Microarray and mass spectroscopy will be used to assess the transcriptomes and proteomes of developing organisms in Biology 356, Developmental Biology lecture and lab. In the early phase (change wording here...training phase is what I mean but doesn't sound right,) yeast will serve as a model for studying the regulation of cell cycle control genes. In the later phase of the grant, evolution of the neural crest will be explored through comparative gene expression patterns in various models such as the zebrafish and sequenced protochordates such as Ciona intestinalis and Oikopleaura. Students will also be able to visualize specific gene expression patterns with in situ hybridization and immunohistochemistry on frozen sections. This work will entail collaboration with and cooperation of labs working with these organisms and with extra slides to share. The yeast microarray data set generated by Biloogy 356 students will be used in a dry lab in Biology 112, Cells, Genes and Inheritance. In this exercise, students will use the data set in GenMAPP to explore strategies for working with huge data sets as well as for studying the central dogma. (More on mass spec and proteomics as I learn more from Chemistry on capabilities, but I could see a similar dry lab/problem space for Cell Phys with emphasis on proteomics but also using the yeast microarray data set.) Biology 356 is an upper-level course with a typical enrollment of 12 to 18 students. Biology 121 is an introductory level course with a typical enrollment of eighty. Peter's plan:

• In the near future we should generate a written synopsis of each particular program component. This mock 'executive summary' should include: 1) Brief summary, 2) Statement of (educational) outcomes, 3) Mode of assessment, 4) Timeframes, and 4) Preliminary budget.